Research Article

Hearing estimated threshold recovery after administering fish oil in sucking periods in n-3 fatty acid-deficient rat pups

Abstract

Background and Aim: Omega 3 of essential fatty acids, especially in fish oil form plays an important role in structural, functional, and biological aspects in body. Omega 3 deficiency can have devastating effects on the nervous system and auditory. This study aimed to evaluate auditory threshold in n-3 fatty acid-deficient rat pups following supplementation of fish oil consumption during the suckling period.
Methods: One sources of omega 3 fatty acid were fed to rat pups of n-3 polyunsaturated fatty acids (PUFA)-deficient dams to compare auditory thresholds in 2, 4, 8, 12 and 16 kHz by auditory brainstem response (ABR) among this group, those with defects with placebo, and control groups. The pups' in supplement group were orally fed 5 mol/g weight of fish oil between the ages of 5 and 21 days.
Results:Results showed significant differences in auditory threshold estimation in 2,4,8,12 and 16 kHz in ABR between study groups. The group with n-3 PUFA deficient with placebo showed a significant increase of the parameters indicated as p<0.05. Moreover a lower threshold (better) in n-3 PUFA deficient with the fish oil in comparison with n-3 PUFA deficient with placebo (p<0.05).
Conclusion: The result of study showed effect of omega 3 deficiency on auditory during pregnancy and lactation period and compensation neural auditory threshold in n-3 fatty acid-deficient rat pups following a supplementation of fish oil consumption during the suckling period.

1. Rizos EC, Ntzani EE, Bika E, Kostapanos MS, Elisaf MS. Association between omega-3 fatty acid supplementation and risk of major cardiovascular disease events: a systematic review and meta-analysis. JAMA. 2012; 308(10):1024-33.
2. Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med (Maywood). 2008;233(6):674-88.
3. Innis SM. Dietary (n-3) fatty acids and brain development. J Nutr. 2007;137(4):855-9.
4. Church MW, Jen KL, Stafferton T, Hotra JW, Adams BR. Reduced auditory acuity in rat pups from excess and deficient omega-3 fatty acid consumption by the mother. Neurotoxicol Teratol. 2007;29(2):203-10.
5. Haubner LY, Stockard JE, Saste MD, Benford VJ, Phelps CP, Chen LT, et al. Maternal dietary docosahexanoic acid content affects the rat pup auditory system. Brain Res Bull. 2002;58(1):1-5.
6. Haubner L, Sullivan J, Ashmeade T, Saste M, Wiener D, Carver J. The effects of maternal dietary docosahexaenoic acid intake on rat pup myelin and the auditory startle esponse. Dev Neurosci. 2007;29(6):460-7.
7. Saste MD, Carver JD, Stockard JE, Benford VJ, Chen LT, Phelps CP. Maternal diet fatty acid composition affects neurodevelopment in rat pups. J Nutr. 1998;128(4):740-3.
8. Church MW, Jen KL, Dowhan LM, Adams BR, Hotra JW. Excess and deficient omega-3 fatty acid during pregnancy and lactation cause impaired neural transmission in rat pups. Neurotoxicol Teratol. 2008;30(2):107-17.
9. Stockard JE, Saste MD, Benford VJ, Barness L, Auestad N, Carver JD. Effect of docosahexaenoic acid content of maternal diet on auditory brainstem conduction times in rat pups. Dev Neurosci. 2000;22(5-6):494-9.
10. Harauma A, Salem N Jr, Moriguchi T. Repletion of n-3 fatty acid deficient dams with alpha-linolenic acid: effects on fetal brain and liver fatty acid composition. Lipids. 2010;45(8):659-68.
11. Kimura F, Ito S, Endo Y, Doisaki N, Koriyama T, Miyazawa T, et al. Supplementation of DHA-rich microalgal oil or fish oil during the suckling period in mildly n-3 fatty acid-deficient rat pups. Lipids. 2011;46(12):1101-10.
12. Sarda N, Gharib A, Moliere P, Grange E, Bobillier P, Lagarde M. Docosahexaenoic acid (cervonic acid) incorporation into different brain regions in the awake rat. Neurosci Lett. 1991;123(1):57-60.
13. Gopinath B, Flood VM, Rochtchina E, McMahon CM, Mitchell P. Consumption of omega-3 fatty acids and fish and risk of age-related hearing loss. Am J Clin Nutr. 2010;92(2):416-21.
14. Salvati S, Attorri L, Di Felice M, Campeggi LM, Pintor A, Tiburzi F, et al. Effect of dietary oils on brain enzymatic activities (2′-3′-cyclic nucleotide 3′-phosphodiesterase and acetylcholinesterase) and muscarinic receptor sites in growing rats. J Nutr Biochem. 1996;7(2):113-7.
Files
IssueVol 24 No 1 (2015) QRcode
SectionResearch Article(s)
Keywords
Fish oil auditory thresholds n-3 fatty acid-deficiency rat pup auditory brainstem response
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Rahimi V, Farahani S, Jalaie S, Saeidpour A. Hearing estimated threshold recovery after administering fish oil in sucking periods in n-3 fatty acid-deficient rat pups. Aud Vestib Res. 2015;24(1):17-24.