Brief Report

Preliminary normative variation of auditory P300 parameters in adult individuals


Background and Aim: Auditory P300 is an event-related potential. Cognitive factors like attention are involved in the generation of P300. It seems that normative variation of P300 is necessary for clinical purposes. Thus, the current study was designed to establish preliminary normative variation of P300 amplitude and latency at Fz and Cz sites in adults.
Methods: This cross-sectional study was performed on 20 right-handed volunteers aged 18 to 33 years. P300 was recorded monaurally with two channels at Fz and Cz placements. Two tone bursts of 1000Hz and 2000Hz were used as frequent and target stimuli, respectively.
Results: The mean values of P300 amplitude and latency at Cz were 7.43±2.61 µv and 325.19±21.34 ms in the right ear and 7.38±2.73µv and 320.29±21.56 ms in the left ear, respectively. At Fz, the mean values of P300 amplitude and latency were 5.34±1.74 µv and 330.09±25.58 ms in the right ear and 5.67±2.30 µv and 329.52±29.25 ms in the left ear, respectively. The differences between the ears at Cz and Fz were not statistically significant (p˃0.05).The mean value of amplitude of P300 was significantly greater at Cz than Fz (p=0.001) although the difference in latency was not statistically significant between Cz and Fz (p˃0.05).
Conclusion: Amplitude of P300 was greater at Cz than Fz although latency was not different. Based on these findings, amplitude and latency values can be probably used for clinical purposes to assess auditory disorders.

1. Ruth RA, Lambert PR. Auditory evoked potentials. Otolaryngol Clin North Am. 1991;24(2):349-70.
2. Huang WJ, Chen WW, Zhang X. The neurophysiology of P300--an integrated review. Eur Rev Med Pharmacol Sci. 2015;19(8):1480-8.
3. Sutton S, Braren M, Zubin J, John ER. Evoked-potential correlates of stimulus uncertainty. Science. 1965;150(3700):1187-8.
4. Hall JW. New handbook of auditory evoked responses. 1st ed. Boston: Pearson; 2007.
5. Linden DE. The p300: where in the brain is it produced and what does it tell us? Neuroscientist. 2005;11(6):563-76.
6. Polich J. P300 in clinical applications: meaning, method, and measurement. American Journal of EEG Technology. 1991;31(3):201-31.
7. Wronka E, Kaiser J, Coenen AM. Neural generators of the auditory evoked potential components P3a and P3b. Acta Neurobiol Exp (Wars). 2012;72(1):51-64.
8. Tarkka IM, Stokic DS. Source localization of P300 from oddball, single stimulus, and omitted-stimulus paradigms. Brain Topogr. 1998;11(2):141-51.
9. Simons CJ, Sambeth A, Krabbendam L, Pfeifer S, van Os J, Riedel WJ. Auditory P300 and N100 components as intermediate phenotypes for psychotic disorder: familial liability and reliability. Clin Neurophysiol. 2011;122(10):1984-90.
10. Steinschneider M, Kurtzberg D, Vaughan HG. Event-related potentials in developmental neuropsychology. In: Rapin I, Segalowitz SJ, editors. Handbook of neuropsychology: vol. 6 child neuropsychology. 1st ed. Amsterdam: Elsevier; 1992. p. 239-99.
11. Polich J, Howard L, Starr A. Stimulus frequency and masking as determinants of P300 latency in event-related potentials from auditory stimuli. Biol Psychol. 1985;21(4):309-18.
12. Duarte JL, Alvarenga Kde F, Banhara MR, Melo AD, Sás RM, Costa Filho OA. P300-long-latency auditory evoked potential in normal hearing subjects: simultaneous recording value in Fz and Cz. Braz J Otorhinolaryngol. 2009;75(2):231-6.
13. Frizzo ACF, Alves RPC, Colafêmina JF. Long auditory evoked potential: comparative study between cerebral hemispheres. Rev Bras Otorhinolaryngol. 2001;67(5):618-25.
14. Massa CG, Rabelo CM, Matas CG, Schochat E, Samelli AG. P300 with verbal and nonverbal stimuli in normal hearing adults. Braz J Otorhinolaryngol. 2011;77(6):686-90.
15. Bennington JY, Polich J. Comparison of P300 from passive and active tasks for auditory and visual stimuli. Int J Psychophysiol. 1999;34(2):171-7.
16. Kimura D. Functional asymmetry of the brain in dichotic listening. Cortex. 1967;3(2):163-78.
17. Li Y, Hu Y, Liu T, Wu D. Dipole source analysis of auditory P300 response in depressive and anxiety disorders. Cogn Neurodyn. 2011;5(2):221-9.
18. Polich J. Updating P300: an integrative theory of P3a and P3b. Clin Neurophysiol. 2007;118(10):2128-48.
19. Mertens R, Polich J. P300 from a single-stimulus paradigm: passive versus active tasks and stimulus modality. Electroencephalogr Clin Neurophysiol. 1997;104(6):488-97.
IssueVol 26 No 2 (2017) QRcode
SectionBrief Report
Adult P300 normal hearing event related potentials

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How to Cite
Najafi S, Rouzbahani M, Heidari F, Hosseini AF. Preliminary normative variation of auditory P300 parameters in adult individuals. Aud Vestib Res. 2017;26(2):112-116.