Tehran University of Medical Sciences Auditory and Vestibular Research 2423-480X 32 2 2022 11 19 114 120 Supriya Mathew Department of Audiology, All India Institute of Speech and Hearing, Mysuru, India Banumathi - Department of Audiology, All India Institute of Speech and Hearing, Mysuru, India Sandeep Kumar Department of Audiology, All India Institute of Speech and Hearing, Mysuru, India Kishore Tanniru Department of Rehabilitation Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia Chandni Jain Department of Audiology, All India Institute of Speech and Hearing, Mysuru, India 2022 09 13 2022 11 05 Background and Aim: Psychoacoustics includes studying the perceived effects of changes in sound intensity, temporal, and frequency aspects that are critical for speech perception. Psycon is one such software used in studies to assess psychoacoustic abilities. Psycon has the potential for wide clinical applications in psychoacoustic research and relies on Auditory syntaX (AUX), a program designed specifically to handle auditory signals. The current study aimed to determine the test-retest reliability of the Psycon application for differential sensitivity measures of frequency, intensity, duration and silence. Methods: The study included 39 participants with normal hearing sensitivity. Psychoacoustic measures, namely, gap detection threshold, duration discrimination threshold, difference limen of intensity, and difference limen of frequency, were used to assess test-retest reliability. The test-retest reliability of all measures was checked in two separate sessions within one day. Results: The reliability of each measure was measured using Cronbach’s alpha. Test-retest reliability of various psychoacoustic tests measured with Psycon ranges from good to excellent. difference limen of frequency had the highest reliability, followed by duration discrimination thresholds, difference limen of intensity, and gap detection thresholds. Conclusion: Psycon appears to be a reliable tool for assessing different psychoacoustic abilities. https://avr.tums.ac.ir/index.php/avr/article/view/1086